How do you put a pipette together?

How do you put a pipette together?

Put the pipette—held at 10–45 degrees—against the wall of the receiving chamber, and smoothly depress the plunger to the first stop. Wait one second and then depress the plunger to the second stop. Slide the tip up the vessel wall to remove the pipette. Allow the plunger to return to its rest position.

How accurate are Eppendorf pipettes?

At 1 000 m above sea level, the volume error of a 100 μL pipette is about –0.3%. When using special tips, that is, tips that significantly differ from standard tips in their geometry, changing the adjustment can improve the dispensing accuracy (systematic error).

How does a pipette work?

Liquid is pulled in and pushed out through the use of displaced air. Each of these pipettes has an internal piston connected to a plunger. For an air displacement pipette, the plunger directly pushes out a volume of air equal to the liquid that will be aspirated.

How do you read a pipette?

Measure using the bottom of the concave surface of the liquid in pipette. This figure illustrates how to read the meniscus on a measuring pipette. Liquid was drawn up to exactly the zero mark and was then dispensed. Reading the value at the bottom of the meniscus shows that 3.19 mL of liquid was delivered.

How do you clean an Eppendorf pipette?

The pipette should be resistant to common cleaning agents and DNA/RNA cleaning solutions to make sure the pipette can be wiped from the outside and rinsed on the inside. Most contamination can be removed by wiping and rinsing.

How do you decontaminate an Eppendorf pipette?

The Eppendorf Research plus and Reference 2 pipettes can be decontaminated either by UV light or by autoclaving the entire instrument. Thus, cleaning following use with infec- tious or contaminated samples is easily possible.

Why can you not manual add the tip to the micropipette with your hands?

Holding the pipette: Do not hold the pipette in your hand while waiting for the centrifuge, cycler, etc. The inside of the pipette will heat up and lead the air cushion to expand resulting in deviations from the set volume when pipetting.

Is the Eppendorf research plus a good pipette?

The Eppendorf Research plus is one of the safest and most ergonomic mechanical pipettes available. Meeting the highest needs of precision and accuracy while providing high flexibility via a large number of variants, the Research plus will become an indispensable companion in your lab.

Why do you need regular maintenance on a pipette?

Regular maintenance and calibration services of pipettes by Eppendorf assures a consistent result quality. Pipettes are precision instruments with parts subject to normal wear and tear leading to imprecision over the time. Therefore, regular maintenance and calibrate a pipette are fundamental to their precision and accuracy.

How long does it take to adjust pipetting volume?

Adjust your pipetting volume in seconds by up to ±2 % for better accuracy when pipetting various difficult liquids like warm, cold, volatile or high density liquids or even when external conditions change (e.g., altitude or use of elongated tips). Change back to factory adjustment easily without calibration.

What kind of calibration is required for a pipette?

*1 10%, 50% and 100% of nominal volumes for variable volume instruments. *3 ISO 17025 accreditation calibration certificate is included in the second calibration after the maintenance.