How does periodic acid Schiff stain work?
How does periodic acid Schiff stain work?
PRINCIPLE: The PAS stain is a histochemical reaction in that the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin-sulfurous acid which form the magenta color. Use skin, aorta or normal liver for positive PAS staining.
What is periodic acid Schiff positive?
Periodic acid–Schiff (PAS) stain highlights the abundant glycogen in the tumor cells; the staining will disappear after diastase treatment. By immunohistochemistry, dysgerminoma cells are positive for PLAP (strong, cytoplasmic staining), SALL4, CD117, and OCT4 (Fig. 16.7).
How do you make periodic acid Schiff stain?
Procedure:
- Deparaffinize and hydrate to water.
- Oxidize in 0.5% periodic acid solution for 5 minutes.
- Rinse in distilled water.
- Place in Schiff reagent for 15 minutes (Sections become light pink color during this step).
- Wash in lukewarm tap water for 5 minutes (Immediately sections turn dark pink color).
What PAS stain detects?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
Why is HIO4 called periodic acid?
Periodic acid, or iodic acid is an oxoacid of iodine having chemical formula HIO4 or H5IO6. In dilute aqueous solution, periodic acid exists as discrete hydronium and metaperiodate ions. When more concentrated, orthoperiodic acid, H5IO6, is formed; this dissociates into hydronium and orthoperiodate ions.
What is periodic acid used for?
Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues.
Which is the full form of PAS?
Power-Assisted Steering (PAS) is a system that helps the driver to steer easily by reducing the steering effort of the steering wheel.
What is the correct formula of periodic acid?
HIO₄
Metaperiodic acid/Formula
Is periodic acid strong?
Periodic acid is a strong oxidizing agent, commonly used to oxidize glycols.