What does ATCC screen cell lines for?

01/08/2021 Off By admin

What does ATCC screen cell lines for?

ATCC credible cells lead to incredible research results The cell biology collection includes more than 4,000 continuous cell lines available by species, tissue/disease types, and signaling pathways. The tumor cell and molecular panels for cancer research are annotated with gene mutations and molecular profiles.

What is a research cell line?

A cell line is a collection of cells originating from one cell. Cell lines are typically kept in a growth medium in tubes, flasks, or dishes – called in vitro cell culture. Here, they can continue to divide indefinitely.

Which benchmark cell line verification is recommended by ATCC?

The American National Standards Institute (ANSI) and the American Type Culture Collection (ATCC) provide a documentary standard (ASN-0002) for human cell lines based on the use of short tandem repeats (STR) entitled Authentication of Human Cell Lines: Standardization of STR Profiling.

How do I get a cell line?

The simplest way to create a new cell line is to modify an existing one, a common strategy when an established line already comes close to meeting the requirements. Cells optimized to grow particular viruses or maximize recombinant protein production often come from such modifications.

How do you develop cell lines?

Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability but is time consuming.

What is the purpose of cell lines?

Cell lines have revolutionized scientific research and are being used in vaccine production, testing drug metabolism and cytotoxicity, antibody production, study of gene function, generation of artificial tissues (e.g., artificial skin) and synthesis of biological compounds e.g., therapeutic proteins.

How do you maintain cell lines?

Many continuous mammalian cell lines can be maintained on a relatively simple medium such as MEM supplemented with serum, and a culture grown in MEM can probably be just as easily grown in DMEM or Medium 199. However, when a specialized function is expressed, a more complex medium may be required.

How do you check cell lines?

The main test for authenticating cell lines is short tandem repeat (STR) profiling, the analysis of microsatellite regions of DNA that have variable numbers of repeats and are located throughout the genome.

How do you validate cell lines?

Phenotypic methods, such as cell morphology and growth curve analysis, are perhaps the simplest methods used to validate cell lines.