What is Biexponential display?

01/11/2021 Off By admin

What is Biexponential display?

Biexponential scaling helps visualize data that is compressed against the low x- and y- axes. “Squished” data is easily viewed by adding a section of linear scale to log acquired data. For large values, this biexponential function displays the data with more log-like properties.

What is Biexponential?

Filters. (mathematics) Having two exponents. adjective.

What is a Logicle transformation?

Description. Logicle transformation creates a subset of biexponentialTransform hyperbolic sine transformation functions that provides several advantages over linear/log transformations for display of flow cytometry data.

What is geometric mean in FlowJo?

Geom. Mean—The geometric mean. Can be a more applicable metric for a log-normal distribution. It is always less than or equal to the arithmetic mean. In FlowJo this is calculated as the geometric mean of the graph space to make it usable on data the may include zeros or negative numbers.

What is biexponential decay?

A biexponential fluorescence decay model is normally used to analyze FLIM-FRET data, where a measured lifetime at a given image pixel is assumed to be composed of two different lifetime components: one with FRET and the other without FRET [21], [22].

How do you compensate in FlowJo v10?

1) To initiate creating a new compensation matrix in FlowJo, select the compensation group in the workspace and go to the Workspace ribbon. 2) Click the compensation icon in the Cytometry band of the Tools tab. 3) The compensation interface will launch.

What is the advantage of a Biexponential scale?

By applying a biexponential transform to the data, the scale is compressed in the lower range, typically from 1-10 or 1-100, leading to a more accurate visual representation of fluorescence units in the low range of the scale as compared to the higher range of the scale.

What is Logicle scale?

The logicle scale is the inverse of a modified biexponential function and has the same relation to the modified biexponential function that a logarithmic scale has to its corresponding exponential function.

What does negative MFI mean?

Negative values mean that the equipment s not well calibrated perhaps control antibodies are not appropriate. You may eventually move the axis to the left. The answer is given by assuming that your cells have the marker you are looking for.

What is terminal phase in pharmacokinetics?

Terminal elimination phase: Following the distribution phase, the drug will be eliminated from the central compartment (by the kidneys/liver) causing changes in both amounts of the drug in the body and plasma drug concentration.