What is phenylhydrazine HCl?
What is phenylhydrazine HCl?
Application. Phenylhydrazine hydrochloride is used in the examination of tyrosine phosphorylation of Janus protein tyrosine kinase (JAKs) in the EPO-responsive normal erythroblastoid cells of anemic mice.
What are the composition of phenylhydrazine mixture?
Phenylhydrazine is prepared by oxidizing aniline with sodium nitrite in the presence of hydrogen chloride to form the diazonium salt, which is subsequently reduced using sodium sulfite in the presence of sodium hydroxide to form the final product.
What is the structure of phenylhydrazine?
What is the Colour of phenylhydrazine?
Phenylhydrazine (CAS No. 100-63-0) exists as yellow to pale brown crystals or as a yellowish oily liquid. It is sparingly soluble in water and is miscible with other organic solvents.
What is phenylhydrazine test?
Osazone test is a chemical test used to detect reducing sugars. This test even allows the differentiation of different reducing sugars on the basis of the time of appearance of the complex. This test is also termed Phenyl hydrazine test based on the reagent used for this test.
What happens when glucose is treated with phenylhydrazine?
When glucose reacts with phenylhydrazine, the products glucosazone, ammonia, and water form.
What is the principle of phenylhydrazine test?
Principle of Osazone Test The reagent for this test consists of phenylhydrazine in acetate buffer. This test is based on the fact that carbohydrates with free or potentially free carbonyl groups react with phenylhydrazine to form osazone.
Does phenylhydrazine react with glucose?
D-glucose reacts with phenylhydrazine to give glucosazone. The same product is obtained from fructose and mannose.
How many Aldohexoses are there?
In the linear form, there are 16 aldohexoses and eight 2-ketohexoses, stereoisomers that differ in the spatial position of the hydroxyl groups. These species occur in pairs of optical isomers.
What kind of solid is phenylhydrazine hydrochloride?
Phenylhydrazine hydrochloride is a white to tan solid with a weak aromatic odor. (EPA, 1998) Phenylhydrazine hydrochloride is a hydrochloride resulting from the reaction of equimolar amounts of phenylhydrazine and hydrogen chloride. It contains a phenylhydrazine.
Why does phenylhydrazine not volatilize in moist soil?
Phenylhydrazine is not expected to volatilize from moist soil surfaces (SRC)because cations do not volatilize and the Henry’s Law constant of the free base is estimated as 2.9X10-8 atm-cu m/mole (SRC), calculated from experimental values for vapor pressure, 0.026 mm Hg (5), and water solubility, 1.27X10+5 mg/L (6).
How long does phenylhydrazine stay in the body?
Evidence from toxicokinetic and toxicity studies and from human experience indicates that phenylhydrazine is well absorbed by the inhalation, oral, and dermal routes in animals and humans. In rabbits, 30-60% of an oral dose of phenylhydrazine was excreted in the urine in 48 hr and (40-60% in 4 days).
How does phenylhydrazine interact with cytochrome P-450?
Evidence from a number of studies in vitro and in vivo suggests that phenylhydrazine interacts with hemoglobin and cytochrome P-450 in an oxidation reaction, resulting in the generation of destructive free radicals, which are responsible for subsequent hemolysis.