What is transfection with siRNA?

08/27/2019 Off By admin

What is transfection with siRNA?

Small interfering RNA (siRNA), also known as silencing RNA, is a double-stranded segment of RNA that can perform various functions in a biological system. A siRNA transfection is the insertion of siRNA into a cell, a process that can be invaluable to gene silencing experiments.

What is Amaxa?

The Amaxa Nucleofector technology is a transfection system designed to transfer genes into cells quickly and efficiently. The technology is based on electroporation. Each cell type is introduced to the DNA (or RNA) in an optimized Nucleofector solution that has been designed for that particular cell type.

What does a Nucleofector do?

It is a powerful tool for transfecting large DNA fragments and achieving good transfection efficiencies in cell lines. The Nucleofector® Technology uses a specific combination of optimized electrical parameters and cell type-specific solutions which enables transfer of a molecule directly into the cells’ nucleus.

How does siRNA transfection work?

These siRNAs are derived from processing of the dsRNA by an RNase III-like enzyme. The mechanism involves the recruitment of siRNAs into a multi-protein complex known as RNA Induced Silencing Complex (RISC), which interacts with the target RNA to mediate cleavage in a catalytic fashion.

What is the purpose of siRNA transfection?

Small interfering RNA (siRNA) is a 20-28 nucleotide double stranded RNA (dsRNA) molecule often referred to as “silencing RNA” . siRNA is used in the gene silencing (or RNA Interference, RNAi) technique to suppress gene expression.

What is transfection method?

Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment.

How do you increase siRNA transfection efficiency?

9 Tips for Optimal siRNA Transfection

  1. Use the most appropriate siRNA concentration.
  2. Prepare a suitable siRNA stock solution.
  3. Transfect healthy cells.
  4. Check serum quality.
  5. Know the target gene in and out.
  6. Always use positive and negative controls.
  7. Follow up the transfection reagent protocol.